null adenovirus  (Vector Biolabs)

Journal: bioRxiv

Article Title: ATGL-catalyzed lipid catabolism promotes DNA repair

doi: 10.64898/2026.04.03.716381

Figure Lengend Snippet: A) AML12 cells transduced with ATGL or GFP control adenoviruses were treated with 30 μM etoposide for the indicated times and assayed for γH2Ax. B) Quantification of A. *p<0.05, **p<0.01. C) AML12 cells transduced with ATGL or GFP control adenoviruses were irradiated (10 Gy) and harvested at the indicated times post-IR. D) Quantification of C. **p<0.01. E) Primary MEFs were isolated from WT or ATGL knock-in (AKI) mice, followed by etoposide incubation for 6 hours. Immunofluorescence was performed for γH2Ax, and cells were imaged using the Agilent Gen5 Cytation. Representative γH2Ax images and foci distribution are shown. F) Cells were treated with DGAT inhibitors for 24 hours, followed by ATGL adenoviral transduction, followed by 24 hours of etoposide treatment. Western blotting for γH2Ax, β-actin, and ATGL. G) AML12 cells were treated as in F. The Comet assay was performed on cells after 24 hours of etoposide. Comets were quantified with OpenComet software. A minimum of 180 comets were quantified per condition. Statistics: one-way ANOVA with Tukey’s post hoc test. ****p<0.0001. H) γH2Ax results and quantification from tissues harvested from E2A Cre-ATGL (AKI) mice irradiated with 7.5 Gy + 4-hour recovery. Mice were of mixed sexes, n=4 mice. P-values from one-way ANOVA are displayed

Article Snippet: For adenoviral transductions, ATGL (Lot: 20160819, mouse ATGL) was obtained from VectorBiolabs, TrackGFP (adGFP) was obtained from UNC Gene Therapy Center, and Null adenovirus was obtained from VectorBiolabs (#1300).

Techniques: Transduction, Control, Irradiation, Isolation, Knock-In, Incubation, Immunofluorescence, Western Blot, Single Cell Gel Electrophoresis, Software

Journal: bioRxiv

Article Title: Progenitor Diversity and Architecture of the Human Ganglionic Eminences Shaping the Basal Ganglia

doi: 10.64898/2025.12.31.697063

Figure Lengend Snippet: A) Experimental scheme of organoid culture. B) Example immunostaining of H28126 line at DIV25. Scale bar is 500mm. C) Quantification using Imaris software of NKX2.1 protein expression at DIV21 (3 cell lines). D) ScRNA-seq analysis of MGE organoids with UMAP clustering revealing 29 distinct cell clusters based on principal component analysis (PCA). E) Proportion of MST divisions relative to non-MST divisions in organoids at DIV25 and DIV47 (3 cell lines). F) Average MST translocation length. G) Immunostaining of organoid slice following live imaging and infection with Ad-CMV-GFP.

Article Snippet: Slices were incubated floating in cell culture medium according to age of organoids with 1:1000 Ad-CMV-GFP (vectorbiolabs; #1060) at 37°C for 3-5 days.

Techniques: Immunostaining, Software, Expressing, Translocation Assay, Imaging, Infection